Yes, certainly the stress of loading and unloading has been addressed and some animals adapt to that more easily than others. Other species of animals can't be unloaded because they're in crates. Different species of animals definitely have different needs, and that has all been looked at and evaluated.
Again, the stress of loading them has to be compared to the stress of not loading or unloading them and the stress of prolonged transportation without any access to feed, water, and rest.
The one thing that we have tried to do in these regulations is to align as much as possible the rest period of the animals with the mandatory rest periods that either exist or are coming into place for the drivers.