Sure. In Ontario recently, GO Transit proudly expanded their service to the community of St. Catharines by running GO buses from St. Catharines to Toronto. There was already an existing Greyhound bus service. Again, it's a case of them literally opening up across the street from the Greyhound terminal, and they started cannibalizing Greyhound's business.
On October 19th, 2011. See this statement in context.